The paper introduces SCR-MF, a two‑stage workflow for single‑cell RNA sequencing imputation that first detects dropout events with scRecover and then imputes missing values using the non‑parametric missForest algorithm. Benchmarking on public and simulated datasets shows that SCR‑MF delivers robust, interpretable results that match or surpass existing methods while maintaining biological fidelity. Runtime analysis indicates that SCR‑MF balances accuracy with computational efficiency, making it well suited for mid‑scale single‑cell studies.
By Ali Anaissi, Deshao Liu, Yuanzhe Jia, Weidong Huang, Widad Alyassine, Junaid Akram
arXiv:2607. 29043v1 Announce Type: cross Abstract: Single-cell RNA sequencing (scRNA-seq) has become an essential tool in modern cellular biology, and generating accurate synthetic scRNA-seq data is becoming increasingly important.
By Yu Song, Hao Sun, Ikuko Nishikawa, Yen-Wei Chen
The paper introduces scTrilemma, a latent-bottleneck variational autoencoder designed to address the representation trilemma in single‑cell RNA‑seq data: preserving biological identity and state, remaining robust to nuisance context, and retaining gene‑level variation for expression analysis. scTrilemma routes expression‑derived variation to the embedding, decoder, or prior, gating gene tokens by expression and conditioning the prior on unlabeled pseudo‑bulk context, all under a single reconstruction objective without target annotations. In zero‑shot evaluations on successive CZ CELLxGENE Census releases, scTrilemma simultaneously satisfies all three demands, maintaining biological state, differential‑expression, and pathway structure across multiple disease settings, and latent interventions show context can be removed with minimal impact on other demands.
By Yunhak Oh, Yoonho Lee, Junseok Lee, Namkyeong Lee, Sang-Yeon Hwang, Yinhua Piao, Hyomin Kim, Seonghwan Kim, Jaechang Lim, Woo Youn Kim, Sungsoo Ahn, Chanyoung Park
arXiv:2606. 26563v1 Announce Type: cross Abstract: Single-cell studies require analysts to convert raw measurements into specific biological claims through multi-step workflows and integration of metadata, assay context, and auxiliary evidence.
By Ian Diks, Zhen Yang, Arjun Banerjee, Tim Proctor, Kenny Workman
The study evaluated nine transcriptomic models—five bulk RNA‑seq and four single‑cell RNA‑seq—designed to predict response to immune checkpoint inhibitors. Across independent datasets, bulk models performed near chance while single‑cell models offered only modest gains, and pathway analyses revealed inconsistent biomarker signals. The results highlight the limited cross‑cohort robustness and biological consistency of current transcriptomic ICI predictors.
By Yuheng Liang, Lucy Chhuo, Ahmadreza Argha, Nona Farbehi, Lu Chen, Roohallah Alizadehsani, Mehdi Hosseinzadeh, Min Yang, Thantrira Porntaveetusm, Youqiong Ye, Hamid Alinejad-Rokny
arXiv:2607. 23821v1 Announce Type: new Abstract: Identifying therapeutic target genes from single-cell RNA sequencing (scRNA-seq) data remains a fundamental challenge in translational biology.
By Shuyu Chen, Chen Zhu, Ye Zhang, Yang Li, Qiqi Xie, Haohan Wang
arXiv:2603. 11872v3 Announce Type: replace-cross Abstract: Translating single-cell RNA sequencing (scRNA-seq) data into mechanistic biological hypotheses remains a critical bottleneck, as agentic AI systems lack direct access to transcriptomic representations while expression foundation models remain opaque to natural language.
By Omar Coser
arXiv:2606. 09558v1 Announce Type: cross Abstract: Motivation: Transformer-based models are increasingly applied to large-scale single-cell transcriptomics, showing strong performance through self-supervised learning on millions of cells.
By Mikele Milia, Louis Fabrice Tshimanga, Henning Mueller, Manfredo Atzori, Barbara Di Camillo
scAnyOmics is a generative framework that distills knowledge from a frozen single-cell foundation model into a multimodal student model, integrating scRNA‑seq, protein‑marker measurements, and scATAC‑seq into a shared representation. It enables cross‑modal prediction and reconstruction of missing modalities, and was evaluated on 690,880 cells from six studies, outperforming baseline methods on held‑out donor data. The framework also supports applications such as cell‑type annotation, perturbation‑response prediction, and biological age estimation, providing a unified approach for integrating incomplete single‑cell multi‑omics data and transferring pretrained biological knowledge.
By Zhaoqi Song, Ye Du, Yusuke Iwasawa, Yutaka Matsuo, Qian Niu
arXiv:2601. 14653v3 Announce Type: replace Abstract: Missing data in single-cell sequencing datasets poses significant challenges for extracting meaningful biological insights.
By Yuyu Liu, Jiannan Yang, Ziyang Yu, Weishen Pan, Fei Wang, Tengfei Ma
arXiv:2611.08086v1 Announce Type: new
Abstract: Modeling gene regulation in single cells requires jointly representing DNA sequence, chromatin accessibility, and gene expression across regulatory sca...
By Kangjie Zheng, Amirhossein Vahidi, Liying Jin, Joseph Clarke, Vijaya Baskar MS, Connor Rogerson, Mengji Zhang, Muzlifah Haniffa, Anshul Kundaje, Mo Lotfollahi
arXiv:2512. 17678v2 Announce Type: replace-cross Abstract: Selecting compact and informative gene subsets from single-cell transcriptomic data is essential for biomarker discovery, improving interpretability, and cost-effective profiling.
By Daphn\'e Chopard, Jorge da Silva Gon\c{c}alves, Irene Cannistraci, Thomas M. Sutter, Julia E. Vogt