FPicker is a topology-guided framework for filament tracing in low‑signal Cryo‑EM images. It combines a center‑endpoint representation with an open‑curve evolution module to model non‑cyclic connectivity, overcoming limitations of pixel‑wise segmenters, box‑based detectors, sequential trackers, and traditional active contours. On simulated benchmarks, FPicker improves mean spatio‑angular precision by over 40% and reduces topological gap rates by more than 60% under extreme noise, and it achieves state‑of‑the‑art performance on real EMPIAR data after fine‑tuning.
By Tingyin Zhao, Mingtao Huang, Yuan Shen
FPicker is a topology‑guided framework for filament tracing in low‑signal Cryo‑EM images. It combines a center‑endpoint representation with an open‑curve evolution module to model non‑cyclic connectivity, overcoming limitations of pixel‑wise segmenters, box‑based detectors, sequential trackers, and traditional active contours. On simulated benchmarks, FPicker improves mean spatio‑angular precision by over 40 % and reduces topological gap rates by more than 60 % under extreme noise, and it achieves state‑of‑the‑art performance on real EMPIAR data after fine‑tuning.
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