The article presents a systematic meta-algorithm for spawning and aggregating multi-class cryo-EM reconstruction jobs, formalizing iterative classification and filtering strategies used by practitioners. It claims to be the first method capable of ab initio reconstruction on datasets with dozens of distinct species, achieving 97% accuracy on a 45-class subset of Tomotwin-100 and 75% on the full dataset, and successfully recovering ribosomal assembly states from an unfiltered experimental cryo-EM dataset. The approach scales with compute resources and aims to underpin automated cryo-EM workflows in contemporary experimental settings.
By Alkin Kaz, Arda Kaz, Ellen D. Zhong
We describe a systematic approach for spawning and aggregating multi-class cryo-EM reconstruction jobs. This approach formalizes standard ad hoc strategies of iterative classification and filtering ty...
arXiv:2610.01358v1 Announce Type: cross
Abstract: Single-particle cryo-electron microscopy (cryo-EM) has become a widely adopted technique for biomolecular structure determination. The conventional c...
By Advaith Maddipatla, M\"art-Erik M\"aeots, Marco Pegoraro, Nikolaus Dr\"ager, Roberto Covino, Sanketh Vedula, Martin Pacesa, Alex M. Bronstein
arXiv:2606. 31332v1 Announce Type: new Abstract: Protein automodeling from cryo-EM density maps faces unique challenges in enforcing physicochemical validity and managing conformational heterogeneity.
By Minzhang Li, Mingrui Li, Weichen Qin, Qihe Chen, Sixian Shen, Yuan Pei, Jiakai Zhang, Jingyi Yu
arXiv:2606. 10255v1 Announce Type: cross Abstract: Cryo-electron tomography (cryoET) has emerged as a powerful tool in structural and cellular biology by enabling direct visualization of macromolecular structures within intact cells, thereby linking molecular architecture to cellular organization in a native context.
By Jonathan Schwartz, Utz Heinrich Ermel, C. Braxton Owens, Zhuowen Zhao, Ariana Peck, Gus L. W. Hart, Grant J. Jensen, Bridget Carragher, Dari Kimanius
arXiv:2609.14097v1 Announce Type: cross
Abstract: Subtomogram classification in cryo-electron tomography (cryo-ET) is a challenging problem due to the scarcity of labeled examples. While cryo-ET simu...
By Siddhant Bharadwaj, Ashish Vashist, Rashi Singh, Pranav Vinodh, Nishanth Artham, Runmin Jiang, Xingjian Li, Min Xu
The paper introduces CARNIVAL, a model for protein annotation in cryo-electron tomography (cryo-ET) volumes that leverages simulated data and a forward model to generate domain‑specific augmented paired views for self‑supervised training. By incorporating simulation‑derived protein positions and identities into the architecture and loss function, the model localises semantic information at protein locations. CARNIVAL is evaluated on real tomograms without finetuning and outperforms a state‑of‑the‑art contrastive model that lacks forward‑model paired views or privileged information.
By Bogdan Toader, Kiarash Jamali, Tanmay A. M. Bharat, Sjors H. W. Scheres
FPicker is a topology-guided framework for filament tracing in low‑signal Cryo‑EM images. It combines a center‑endpoint representation with an open‑curve evolution module to model non‑cyclic connectivity, overcoming limitations of pixel‑wise segmenters, box‑based detectors, sequential trackers, and traditional active contours. On simulated benchmarks, FPicker improves mean spatio‑angular precision by over 40% and reduces topological gap rates by more than 60% under extreme noise, and it achieves state‑of‑the‑art performance on real EMPIAR data after fine‑tuning.
By Tingyin Zhao, Mingtao Huang, Yuan Shen
FPicker is a topology‑guided framework for filament tracing in low‑signal Cryo‑EM images. It combines a center‑endpoint representation with an open‑curve evolution module to model non‑cyclic connectivity, overcoming limitations of pixel‑wise segmenters, box‑based detectors, sequential trackers, and traditional active contours. On simulated benchmarks, FPicker improves mean spatio‑angular precision by over 40 % and reduces topological gap rates by more than 60 % under extreme noise, and it achieves state‑of‑the‑art performance on real EMPIAR data after fine‑tuning.
arXiv:2604.10766v5 Announce Type: replace
Abstract: Open-set 3D macromolecule detection in cryogenic electron tomography eliminates the need for target-specific model retraining. However, strict VRAM...
By Ming-Yang Ho, Alberto Bartesaghi
arXiv:2606. 00955v1 Announce Type: new Abstract: Despite the growing availability of cryo-electron microscopy (cryo-EM) density maps, effectively leveraging them for protein representation remains challenging.
By Dan Luo, Xuan Lin, Peng Zhou, Junwen Zhu, Tengfei Ma, Xiangxiang Zeng, Yiping Liu
Atelier is a self‑supervised framework that uses a transformer‑based hypernetwork to generate implicit neural representations (INRs) for cryo‑EM maps, enabling efficient, scale‑agnostic, coordinate‑conditioned feature extraction. Trained on 5,439 maps from the Electron Microscopy Data Bank, the pretrained INR provides continuous local feature fields that can be used as auxiliary channels for a 3D nested U‑Net, improving voxel‑level property prediction across eight tasks compared to a volume‑only baseline. The approach demonstrates that amortized INRs can serve as a geometry‑aware primitive for large‑scale cryo‑EM analysis.
By Phillip Lo, Sudarshan Babu, Dari Kimanius, Aly A. Khan